Learning outcomes of the course unit
To acquire the theoretical knowledge needed to understand and put into practice the basic techniques to be used in the Pathological Anatomy laboratories, including, among the others: tissue processing for histopatology, sectioning and staining (histochemical, immunohistochemical) of histological slides, application of molecular biology techniques to the study of pathological processes
Knowledge of the basis of histology.
Course contents summary
The course will be mainly focused on the morphologic and molecular techniques applied to the study of pathologic tissues
Organization of the histopathology laboratory
Tissue sampling, fixation and processing
Intraoperative frozen sections examination and cryotomy
Molecular biology techniques applied to Pathologic Anatomy (Polymerase Chain Reaction, Fluorescent In Situ Hybridization
- Theoretical basis of FISH technology
- FISH applications to the study of numerical and structural chromosomal aberrations
- FISH analysis implications to oncologic treatment with targeted-therapies
- Set-up of a FISH experiment in the laboratory.
AA.VV. Anatomia Patologica: le basi. A cura di L. Ruco, A. Scarpa. UTET Scienze Mediche, Torino, 2007
AA.VV. Manuale di Tecnica Cito-Istologica. A cura di S. Daniel, T. Zanin.
Documentazione Scientifica Editrice (DSE), Bologna, 1997
Printed forms of the Power Point slides presented during the course
Assessment methods and criteria
Final oral exam.