Learning outcomes of the course unit
This course aims to provide the informations and the scientific background to approach correctly the practical aspects in Clinical Biochemistry (also out of conventional laboratories) to figure out the complexity concerning the biological samples and the possible sources of error, with respect to analysis in the sport ambit aiming the prevention of illicit expedients, the athlete health or the role of clinical biochemistry in the monitoring of training.
There are no obligatory prerequisites, but several topics in Clinical Biochemistry are part of an interdisciplinary science, thus the student needs the basic knowledge in Biology, Anatomy, Physiology, Chemistry and Biochemistry
Course contents summary
Laboratory medicine with regard to sport doping prevention. Laboratory tests: definition, typology, request modality. Biological sample: properties and characteristics. Pre-analytical and analytical variabilities affecting the laboratory data. Strategy about laboratory investigation of prohibited substances or methods: direct detection or recognition of biochemical alteration induced on the metabolisms.
Basic biochemical analysis profiles. Markers of organ function or damage mainly with respect to skeletal muscle, liver, kidney and heart.Current waived test. Point of care testing (POCT): use and application. Criteria for test waiving. Quality control in POCT. Implementation and monitoring of POCT. Specimen and its treatment in POCT. Analytical basic principles of prohibited substances belonging to the following classes: stimulants, narcotics, diuretics, peptide hormones, anti-estrogens, masking tools. Analytical basic principles to investigate the following prohibited methods: haematic doping, chemical or physical or pharmacological manipulation, genetic doping.
Laboratory medicine with regard to sport and to the doping prevention.
Laboratory tests: definition, typology, request modality.
Biological sample: properties and characteristics.
Pre-analytical and analytical variables affecting the laboratory data, particular attention will be on the Point of care testing (POCT) instrumentation.
Strategy about laboratory investigation of prohibited substances or methods: direct detection or recognition of biochemical alteration induced on the metabolisms.
Basic biochemical analysis profiles.
Markers of muscle damage.
Meaning of the most common Chemical-Clinical parameters, markers of functionality and/or metabolic, organs and tissue damages.
Biological samples and their treatment.
S.D.Ferrara. Doping Antidoping -Piccin
Spandrio: Biochimica Clinica. Ed. Sorbona, Milano
Assessment methods and criteria
The final oral examination is about the topics of the course; the student needs to demonstrate to have understood and to be able to manage the essential meanings of every section of the program.
The didactic materials (slides, texts besides the suggested ones, different information accessible form the web) used during the lectures, is available for students on request.