BIOTECHNOLOGICAL VACCINES AND VIRAL VECTORS
Learning outcomes of the course unit
Learning on the manipulation and use of lentiviral vectors
Chemistry, biochemistry, molecular biology, microbiology and immunology.
Course contents summary
Second and third generation of letiviral vector.
Employment of lentiviral vectors
Principles and immunological basis of vaccinology. Relevant available
anti-infective vaccines (live-attenuated, killed-inactivated, subunit,
purified or conjugated antigens, toxoids, recombinant) or experimental
(DNA, anti-idiotypic). Biotechnological vaccines and their design:
characteristics and development. Existing biotechnological vaccines
(HBV, HPV, Rotavirus, polysaccharide-conjugated vaccines). Rational
design. Reverse vaccinology. Recombinant bacteriophges and bacteria as
vaccines and vaccine delivery veicles. Plant-based vaccines. Adjuvants
and delivery systems. Viral vectors for gene therapy and immunotherapy.
Retrovirus: Molecular aspects of viral replication. Architecture of following
viral vectors: Oncoretrovirus (MMLV) and Lentivirus. Packaging strategies
and mechanism of targeting and peseudotypization (VSV-G). Cellular
transduction and expression control.
Assessment methods and criteria