BIOMOLECULAR STRUCTURE AND DYNAMICS
Learning outcomes of the course unit
Considering the significant development and relevance of the structural proteomics, this course is designed to give students a basic knowledge of the principles and practise of the most important methods to determine the structure, the conformational stability and dynamics of proteins in solution.
Course contents summary
Forces that determine biomolecular structure. Structure and dynamics of proteins.
Basic principles of spectroscopy. Electromagnetic waves polarization. Interaction of radiation with matter.
Circular dichroism spectroscopy (CD): basic principles and application to the study of biomolecules.
Nuclear Magnetic Resonance (NMR) spectroscopy: basic principles and applications to the study of structure and dynamics of proteins in solution.
Methods to determine the three dimensional structure of protein using NMR data.
Main features of Magnetic Resonance Imaging (MRI).
Fluorescence spectroscopy: basic principles and application to biochemical studies.
All the study materials and supporting web sites are provided by the teacher.
Assessment methods and criteria
The exam consists of an oral examination.