Genome sequencing has generated a huge amount of data which have revolutionised the research approach. the 'omic' concept has been applied to all field of genetic research with the aim to study the whole genome at structural and functional level. the objective of the course is to provide the knowlegde of the basic principle of the new approaches in the field of biological and genetic analysis.
Course contents summary
Definition of what is a genome. Genetic mapping, physical mapping. Genome sequencing. The genomic projects: overview of the main initiatives. The annotation of genomic sequences.
Overview of the most popular molecular markers (RFLP, SSR, AFLP, SNP and others)
Protocols and associated problems
Use of molecular markers in fingerprinting
Use of molecular markers for genetic mapping
Use of molecular markers for “bulk segregant analysis” and “association mapping”
Plant variety fingerprinting, identification and cluster analysis
Locating genes in plants for important traits
Cloning genes in plants
In situ hybridisations
Positional cloning of disease genes
Brown TA, “Genomi” Edises (cap 1-9)
Greg Gibson and Spencer V. Muse,"Indroduzione alla Genomica" - Zanichelli (cap. 1,2,5)
Lewin B, et al. “ Il gene” Zanichelli ( cap. 4, 5, 23)
-Wang D.G, Fan J.B., Siao C.J., Berno A., Young P., Sapolsky R., Ghandour G., Perkins N., Winchester E., Spencer J., Kruglyak L., Stein L., Hsie L., Topaloglou T., Hubbell E., Robinson E., Mittmann M., Morris M.S., Shen N., Kilburn D., Rioux J., Nusbaum C., Rozen S., Hudson T.J., Lipshutz R., Chee M., Lander E.S. Large-scale identification, mapping, and genotypimg of single-nucleotide polymorphisms in the human genome. Science. 1998, 280: 1077-1082
-Rafalski A. Applications of single nucleotide polymorphisms in crop genetics. Current Opinion in Plant Biology. 2002, 5:94-100
-Morgante, M., and A.M. Olivieri. PCR-amplified microsatellites as markers in plant genetics. Plant J. 1993, 3:175-182.
-Hunt D.J., Parkes H.C., Lumley I.D. Identification of the Species of Origin of Raw and Cooked Meat Products Using Oligonucleotides Probes. Food Chem. 1997, 60: 437-442
-Gupta P.K., Roy J.K., Prasad M. Single nucleotide polymorphisms: a new paradigm for molecular marker technology and DNA polymorphism detection with emphasis on their use in plants. Curr.Sci. 2001, 80: 524-535
-Andersen J.R. and Lübberstedt T. Functional markers in plants. Trends in Plant Science. 2003, 8 (11): 554-560