Learning outcomes of the course unit
Objectives of the course :
To provide the second year students with the basic concepts of classical genetics examples of genetic strategies to answer to specific questions.
Each topic will be presented in experimental form, with an attempt to answer to the following questions: what was the reason for the experiment, how was it carried out, what result did it lead to and what is the meaning of the results?
First year Genetics
Course contents summary
General regulatory systems.- Glucose repression in bacteria: physiological analysis and selection of mutants - Control of glucose repression in bacteria: cAMP and CAP- Glucose repression in yeast. Physiological analysis - Genetic control of glucose repression- Strategies to select glucose non-represseible and non-derepressible mutants- Genetic analysis of the selected mutants- Physiological analysis and determination of glucose uptake- Headie-Hofstee plot of the mutants: Identification of low and high affinity transporters- Reversion and suppression. - Search of suppressors to find out new genes. - Petite mutants in yeast. - Genetic and physiological analysis. Non- Mendelian inheritance.
Papers related to the specific subjects will be indicated to the students attending to the course
Teaching methods: Oral and practical lesson
Assessment methods: written exam
Language of instruction: Italiano